Nuclease, S1

I.U.B.: 3.1.30.1

C.A.S.: 37288-25-8

Enzymatic Reaction (image will open in a new window) 

Nuclease S1, isolated from certain Neurospora and Aspergillus species, specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. It is used to eliminate non-annealed polynucleotide tails and hair-pin loops in DNA-RNA or DNA-DNA duplexes in hybridization studies and in genetic recombination experiments.

Molecular weight:

Approximately 32,000 - 36,000 daltons, exists as a monomer. (Vogt, V. 1973).

Optimum pH:

4.0 - 4.6. (Vogt 1973 and Ando 1966).

Activators:

Zn⁺⁺ and/or Ca⁺⁺. (Vogt 1973 and Ando 1966).

Inhibitors:

EDTA, citrate (Vogt 1973 and Ando 1966) and a high concentration of SDS.

Storage:

Store at -20°C.