Worthington Biochemical FAQ

What are the volumes of Hank's, Earle's and Leibovitz L-15 Media that you sell?

Sterile calcium and magnesium free Hank's Balanced Salt Solution, p 7.4 is supplied in the NCIS (Neonatal Cardiomyocyte Isolation System) kit. A 500ml bottle may be purchased, catalog #LK003210.
The NCIS kit also contains a pouch of Leibovitz L-15 Media Powder. Reconstitute the entire contents of the pouch into a beaker containing 800ml of cell culture grade water. Rinse pouce 2-3 times with additional 100ml. Bring total volume to 1 liter and filter through a 0.22 micron pore size filter. The pouch containing Leibovitz L-15 Media powder for 1 liter is catalog #LK003250.
Sterile Earle's Balanced Salt Solution (EBSS) with calcium, magnesium, bicarbonate and phenol red is supplied with the PDS (Papain Dissociation System) kit. A 100 ml bottle may be purchased, catalog #LK003182.