Worthington Biochemical FAQ

What are the basic differences among the four types of collagenase?

CLS-1 Contains average amounts of assayed activities (collagenase, caseinase, tryptic and clostripain). Recommended for epithelial, liver (hepatocytes), lung, fat (adipocytes) and adrenal cell isolations.
CLS-2 Contains greater proteolytic activities, especially clostripain activities. It is generally used for heart (cardiomyocytes), bone, muscle, thyroid, cartilage and endothelial cells.
CLS-3 All activities lower than average. Low caseinase and low proteolytic activity. It is usually used for mammary cells and other soft tissues.
CLS-4 Selected because of low tryptic activity. High collagenase required. It is commonly used for pancreatic islets and other applications where receptor integrity is crucial.