Worthington Biochemical FAQ

What is an acceptable enzyme diluent for Papain (PAP/PAPL)?

A common working solution of papain is 20 u/ml papain in 1mM L-cysteine with 0.5mM EDTA dissolved in balanced salt solution of choice (ie EBSS etc). EDTA, cysteine and dimercaptoethanol are considered stabilizers. Optimum pH range is 6.0-7.0.